龙峰等：highly intensive and long-lasting chemiluminescence “three-in-one” hemin/fe3o4@mos2 hybrid nanozyme powered lab-on-fiber device for ochratoxin a immunoassay
发布时间：2022-01-01
abstract：a novel long-lasting chemiluminescent (cl) “three-in-one” hemin/fe3o4 @mos2 hybrid nanozyme powered lab-on-fiber (cn-lof) device was developed for ochratoxin a (ota) detection. the “three-in-one” nanozymes could catalyze luminol-h2o2 system to produce robust and long-lasting cl under ph range of 6.5–9.0 and temperature range of 0–70 ℃, such excellent catalytic activity was mainly originated from the synergistic effect of mos2, fe3o4 and hemin and the nanozyme’s elegant 2d/3d structure. in the reaction system, the nanozymes could continuously catalyze h2o2 to generate o2•− and 1o2 species, which was the main reason for the production of persistent cl. using ota antibodies labeled nanozyme for cl signal amplification while a reusable functionalized optical fiber as a biorecognition and separation element and also as a cl transducer, the cn-lof could quantify ota with high sensitivity and stability within 20 min. the obtained linear detection range for ota was 0.69–65.85 ng/ml and the lod was 0.16 ng/ml under optimal conditions. the proposed cn-lof method had been successfully applied to the detection of ota in underground water and milk samples, and the detection results were highly consistent with those of lc-ms, demonstrated the cn-lof method had great application potential in the analysis of environmental samples and the detection of foodborne biotoxins.
keywords: nanozyme; long-lasting chemiluminescence; ochratoxin a; lab-on-fiber; immunoassay
原文载于:sensors and actuators: b. chemical2023, (sci, q1, if= 8.4).
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